Inducible expression of immediate early genes is regulated through dynamic chromatin association by NF45/ILF2 and NF90/NF110/ILF3
Fig 5
Reduced NF90/NF110 or NF45 expression attenuated inducible expression of IEG RNAs.
HEK293 D2 or D5 cells were treated without or with doxycycline, then serum-starved for 12 h and treated with PMA (20 ng/ml) for 0, 15, or 30 min. Total RNA was prepared and used for reverse transcription qPCR to detect EGR1, FOS, and JUN mRNAs and pre-mRNAs. ACTB was used for normalization. N = 3 biological replicates; One-way analysis of variance (ANOVA) test followed by post-hoc Student’s t-test corrected by Bonferroni; data reported as mean ± s.e.m of fold-induction compared to 0 min, * P < 0.05, ** P < 0.01.