Inducible expression of immediate early genes is regulated through dynamic chromatin association by NF45/ILF2 and NF90/NF110/ILF3
Fig 1
NF90/NF110 occupancy at promoters of immediate early genes in K562 cells.
(a) Average occupancy profile of NF90/NF110/NF110 at proximal promoters within 1000 bp upstream of transcription start site (TSS) of 49 immediate early genes (IEG), 58 delayed primary response genes (D-PRG), and 26 secondary response gene (SRG) defined by Tullai et al. x-axis: Relative position near TSS. y-axis: Fold-change over input of NF90/NF110/NF110 ChIP-Seq signal. (b-d) Signal tracks of NF90/NF110 chromatin occupancy determined by ChIP-seq at promoters of IEGs EGR1, FOS, and JUN; retrieved from UCSC genome browser [41]. NF90/NF110 ChIP-seq signal (fold change over input) is presented, aligned to peaks called by Irreproducible Discovery Rate (IDR) analysis that measures consistency between replicates. Relative position of the amplicons in the proximal promoter and gene body of each gene used for subsequent ChIP-PCR experiments are indicated.