Pore-forming spider venom peptides show cytotoxicity to hyperpolarized cancer cells expressing K+ channels: A lentiviral vector approach
Fig 6
K+ channel current-dependent cytotoxicity Lv-LaFr26- and Lv-Oxy-2b-transduced cells.
(A, B, and C) Lv-LaFr26, Lv-Oxy-2b, and Lv-mCherry were added to the media of 293T cells that stably express GFP or Kir2.1 and the cell viabilities were measured 72 h after addition. Both Lv-LaFr26 and Lv-Oxy-2b transductions resulted in cytotoxicity only to Kir2.1-expressing cells (A and B). (C) Contrastingly, Lv-mCherry infection did not lead to any cytotoxicity to GFP- or Kir2.1-expressing cells (n = 4). (D) 56–3 cells were incubated with conditioned media from the cells transduced with Lv-mCherry, Lv-LaFr26, and Lv-Oxy-2b. Cell viabilities were measured after 24 h (n = 8, 4, and 4).