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Development of a high-throughput fluorescent no-wash sodium influx assay

Fig 6

Concentration-response curves for the Na_V channel inhibitor tetracaine at hNa_V1.1–1.8 obtained using a no-wash sodium influx assay ANG-2 and the quencher Ponceau 4R.

Preincubation of tetracaine concentration-dependently reduced the fluorescence response caused by addition of veratridine (60 μM; Na_V1.1–1.7) or deltamethrin (150 μM; Na_V1.8) with the following IC₅₀’s: Na_V1.1 52 μM, Na_V1.2 44 μM, Na_V1.3 36 μM, Na_V1.4 13 μM, Na_V1.5 14 μM, Na_V1.6 66 μM, Na_V1.7 21 μM, Na_V1.8 6 μM. Data are presented as mean ± SEM from three wells.

doi: https://doi.org/10.1371/journal.pone.0213751.g006