Development of a high-throughput fluorescent no-wash sodium influx assay
Fig 5
Concentration-response curves for veratridine at hNaV1.1–1.7 and deltamethrin at hNaV1.8 obtained using a no-wash sodium influx assay using ANG-2 and the quencher Ponceau 4R.
Addition of the non-selective NaV channel activator veratridine concentration-dependently increased fluorescence with the following EC50’s: NaV1.1 21 μM, NaV1.2 16 μM, NaV1.3 12 μM, NaV1.4 16 μM, NaV1.5 23 μM, NaV1.6 10 μM, NaV1.7 29 μM. Addition of the NaV channel activator deltamethrin concentration-dependently increased fluorescence at NaV1.8 with an EC50 of 169 μM. Data are presented as mean ± SEM from three wells.