Acetylcholine-treated murine dendritic cells promote inflammatory lung injury
Fig 5
M3 mAChR is involved in ACh-induced activation of DC.
(A-C) DC (1x106 cells/ml) were exposed or not to selective M3 receptor antagonist (Tio 30nM for 30 m) and then were incubated with or without ACh 10−11 M for 18 h. M3 (A) and MHC II IAd (B) expression was determined by flow cytometry and representative histograms of MIF of 5 experiments are shown (mean ± SEM, 2 replicates/experiment). TNF-α production (C) was measured by ELISA (mean ± SEM, n = 4 experiments). *p<0.05, nonparametric Wilcoxon test.