Huntingtin associates with the actin cytoskeleton and α-actinin isoforms to influence stimulus dependent morphology changes
Fig 3
Huntingtin can co-localize to vinculin-positive adhesion structures.
Double-label immunofluorescence with anti-Huntingtin antibody Ab2527 (Green) and anti-Vinculin (Red). (a) In serum starved cells, Huntingtin immunoreactivity occurs along stress fibers leading into large Vinculin-positive adhesion plaques (top row, arrows), from which Huntingtin is excluded. In cells containing less prominent stress fibers (bottom row), small foci of staining for Huntingtin with Ab2527 was present in Vinculin-positive adhesion structures that appeared less well-organized than those depicted in a suggesting immature or developing adhesion plaques (inset). (b) In cells grown with serum with no stress fibers and with focal contacts, Huntingtin labeled with Ab2527 and Vinculin co-localize (arrows in merged). Scale bar in a = 5 μm and applies to all images.