Testing of library preparation methods for transcriptome sequencing of real life glioblastoma and brain tissue specimens: A comparative study with special focus on long non-coding RNAs
Fig 5
The percentage of reads aligned to particular regions in the reference genome.
A) Among CFG libraries, SENSE (LX) libraries contained sequences which on average mapped more to coding regions and less to UTR compared to the other two kits. The number of bases aligning to intronic and intergenic regions ranged from 9.5 to 21.8% and 3.0 to 6.1%, respectively, with no major differences between the three groups of libraries. The highest numbers of bases aligning to ribosomal regions belonged to libraries G2_NB (11.1%), G2_NF (14.0%) and N1_NB (14.7%). B) VB libraries mapped to particular regions in a similar manner to NB libraries, as was expected, with the higher number of bases mapping to UTR compared to coding regions but with lower amount of base reads aligning to ribosomal regions.