AMPA receptor antagonist perampanel affects glioblastoma cell growth and glutamate release in vitro
Fig 3
Glutamate release of glioblastoma and brain metastasis cells.
In subconfluent cell cultures, supernatants (w/o FCS) were collected for a total of 24 hours (± PER) and glutamate levels were determined. Extracellular glutamate levels were normalized to total protein levels of the cells. Data are presented as mean ± SEM (n≥14), *p<0.05 vs. solvent control (Mann-Whitney U test). Multiple comparisons versus control groups (two-way ANOVA with Bonferroni t-test) demonstrated an overall higher glutamate level in the supernatant of glioblastoma cells than in the supernatant of metastasis cells (p<0.001). Additionally, the two-way ANOVA also revealed a significant treatment effect, i.e. PER attenuated extracellular glutamate levels across all cell cohorts (p = 0.046).