In vitro and in vivo properties of the bovine antimicrobial peptide, Bactenecin 5
Fig 5
Exogenous injected Bac5 slows rate of infection with m. marinum in zebrafish embryos.
Two days post-fertilisation casper Tg(mpx:GFP) zebrafish embryos were injected into their HBV with 10 ng Bac5 or mock control along with approximately 250 CFU Mmar expressing DsRed2 for both infection groups. Zebrafish embryos were live-imaged using a fluorescence stereomicroscope and z-stack images of the HBV region acquired at 6 hpi, 24 hpi and 72 hpi. Bacterial burden in the HBV region of M. marinum–infected zebrafish embryos was quantified from fluorescence images using Icy FPC protocol. Sample size (n): 38, 43. Data pooled from three independent experiments is shown. Error bars represent S.E.M. Mann-Whitney test. *p<0.05.