In vitro and in vivo properties of the bovine antimicrobial peptide, Bactenecin 5
Fig 2
Effect of Bac5 on THP-1 cell cytokine transcription during infection.
Fold change of IL-1β, TNF-α and MMP-9 mRNA levels in uninfected (A) and M. marinum-infected (B) resting THP-1 cells following incubation with 15 ng/μl AMPs for 24 h. Fold changes of mRNA levels were assessed by qRT-PCR, normalised to 18S and expressed relative to one control (no treatment) sample. Data pooled from two independent experiments is shown. Each data point represents two treatment wells (n = 12). Error bars represent S.E.M. Kruskal-Wallis with Dunn’s post-test. **p<0.01, *p<0.05.