In vitro and in vivo properties of the bovine antimicrobial peptide, Bactenecin 5
Fig 1
Antibacterial effect of Bac5 against gram-negative and Acid-Fast bacilli.
The active fragment of Bac5 was synthesised to high purity and incubated at different concentrations with mid-log phase bacteria of different species in a sterile solution of RPMI:Water (1:4, v:v). Metabolic activity (A) was calculated using the colorimetric Alamar Blue assay, while bactericidal activity was recorded by colony forming unit (CFU) assay (B). All data were collected at 24 hours, except for M. bovis BCG, where metabolic activity was recorded after 72 hours. Stars denote statistical (p< 0.01) significance from the untreated (zero) control for each species as calculated using Student’s T-test.