Analysis of colony phase variation switch in Acinetobacter baumannii clinical isolates
Fig 5
Outer membrane vesicles released by A. baumannii opaque and translucent variants.
(A) Visualization of OMVs from opaque and translucent phase variants by atomic force microscopy, (B) Cellular uptake of micro vesicles (OMVs) isolated from A. baumannii Ab-Pak-Pesh-37 opaque (upper panel) and translucent (lower panel) phase variants. Scale bar = 5μm, (C) NFKB nuclear translocation and activation in response to OMVs isolated from A. baumannii Ab-Pak-Pesh-37. Scale bar = 5μm, and (D) Quantification of NFKB staining intensity in cell nuclei (n = 20 cells). Groups were compared using paired t-test (Microsoft Excel). Groups were compared using paired t-test (Microsoft Excel). p values of <0.01 ** or <0.05 * were considered statistically significant.