Sphingosine 1-phosphate (S1P) reduces hepatocyte growth factor-induced migration of hepatocellular carcinoma cells via S1P receptor 2
Fig 1
Effects of HGF on the phosphorylation of p38 MAPK and MYPT-1 in HuH7 cells.
The cultured cells were stimulated with 30 ng/ml HGF for the indicated periods. The cell extracts were then subjected to SDS-PAGE with subsequent Western blot analysis using antibodies against phospho-specific p38 MAPK, phospho-specific MYPT-1 or GAPDH.