Improved multilineage human hematopoietic reconstitution and function in NSGS mice
Fig 6
Rapid T cell differentiation in NSGS mice.
Representative flow plots from week 16 engrafted mice showing lineage distribution in A) spleen and B) PB. C) Flow plots showing potential T cell contamination in CD34+ isolations that was removed by FACS sorting CD34+CD3- cells. D) Kinetics of total human, B, myeloid, and T cells engraftment in the PB of NSG and NSGS mice engrafted with the same FACS sorted UCB CD34+CD3- samples. E) Thymus FACS analysis shows normal double positive primitive T cells. F) PB CD4 and CD8 frequencies among CD3+ T cells. G) Spleen Tregs were enumerated. A representative flow plot example is shown in H and the data is displayed as percentage of CD4 cells in I. J) RA/RO ratios in spleen CD3+ cells. Panels E-J were completed 15 weeks after engraftment. Asterisks indicate p value less than 0.05 by Student’s T Test.