Improved multilineage human hematopoietic reconstitution and function in NSGS mice
Fig 1
Four strain comparison of engraftment.
A) Total human engraftment (CD45+) in the BM of mice at 8 and 16 weeks. B) Lineage composition of the human grafts from the BM, PB and spleen. B cells (CD19+), myeloid cells (CD13+ and CD33+), and T cells (CD3+) were assessed within the CD45+ population. C) PB CD45+ levels in mice engrafted with 6 unique UCB CD34+ preparations 6–8 weeks after engraftment. D) PB grafts from mice in “C” were plotted against the BM grafts from the same mice. The slopes of the trendlines were judged to be different by linear regression analysis, Prism 7 software, p = 0.0023. E) Lineage analysis of BM and PB for the grafts in “C”. F) Wright Giemsa stained cytospins of FACS sorted CD45+ cells from BM and spleen. G) Human cytokine serum concentrations in humanized mice. H) Flow cytometric analysis of BM grafts at 16 weeks from some mice included in experiments presented in panels A and B. I) Average total BM WBCs in 1 femur and 1 tibia (combined) were determined for NSG and NSGS mice at 15 weeks of engraftment. Total cellularity was not significantly different. Asterisks indicate p value less than 0.05 by Student’s T Test.