Use of a sequential high throughput screening assay to identify novel inhibitors of the eukaryotic SRP-Sec61 targeting/translocation pathway
Fig 1
Scheme of the primary and the secondary screening procedures.
In the primary screen (upper panel), construct CRF1R.GFP was used, a GFP-tagged GPCR possessing a cleavable signal peptide which uses the SRP-Sec61 targeting/translocation pathway. The secondary screen (lower panel) was performed with unfused, soluble GFP which does not use the SRP-Sec61 targeting/translocation pathway. See the text for details.