Increased triacylglycerol - Fatty acid substrate cycling in human skeletal muscle cells exposed to eicosapentaenoic acid
Fig 5
Myotubes were incubated with PA or EPA (100 μM) for 24 h and then harvested for RNA isolation. Affymetrix human NuGO GeneChip arrays was used to measure gene expression and analysis performed in Partek Genomics Suite 6.6 software. GO enrichment analysis revealed that the GO terms”Neutral lipid metabolic process” (GO:0006638, upper 3) and”Regulation of lipid storage” (GO:0010883, lower 4) were changed significantly (p<0.05) by EPA vs. PA. They are presented with % of genes up- or down-regulated in each child GO term and genes are presented in S1 Table. EPA, eicosapentaenoic acid; PA, palmitic acid.