Increased triacylglycerol - Fatty acid substrate cycling in human skeletal muscle cells exposed to eicosapentaenoic acid
Fig 4
Mitochondrial function after preincubation with fatty acids.
Human skeletal muscle cells were grown in 96-well Seahorse tissue culture plates, and treated with 100 μM of PA or EPA for 24 h, before measurement of oxygen consumption rate (OCR) with the Seahorse XF96e analyzer. (A) OCR time curve from an experiment with one of two donors. (B) OCR parameter calculations by the Seahorse XF Mito Stress Test Report Generator from experiments with two different donors (n = 8). Results represent mean ± SEM. *p<0.05 for EPA vs. PA (t-test). EPA, eicosapentaenoic acid; PA, palmitic acid; Oligo, oligomycin; FCCP, carbonyl cyanide-p-trifluoromethoxyphenylhydrazone; Rot, rotenone, AA, antimycin A.