Increased triacylglycerol - Fatty acid substrate cycling in human skeletal muscle cells exposed to eicosapentaenoic acid
Fig 1
Accumulation and distribution of oleic acid in myotubes when co-incubated with fatty acids.
Human myotubes were grown and differentiated in 96-well ScintiPlate or 12-well tissue culture plates. On day 6 of differentiation the myotubes were treated with a mixture of 100 μM fatty acids for 24 h. The mixture was trace amounts of [14C]OA (9 μM) and non-labeled PA (16:0) or EPA (20:5, n-3) (91 μM). (A) Cell-associated radioactivity was measured during 24 h by SPA. Real-time accumulation of radiolabel was monitored as described in Methods. Results represent mean ± SEM (nmol/mg protein) for n = 3 donors. Significant increase for EPA vs. PA (all-over effect). p<0.05 for EPA vs. PA, LMM statistical test (SPSS). (B-C) Lipids were separated by thin layer chromatography and quantified by liquid scintillation. Results are shown as mean ± SEM for absolute values, nmol/mg protein (B) and related to PA, % (C) from 4 individual experiments. *p<0.05 for EPA vs. PA (t-test). EPA, eicosapentaenoic acid; PA, palmitic acid; SPA, scintillation proximity assay; LMM, linear mixed model; FFA, free fatty acid; DAG, diacylglycerol; TAG, triacylglycerol; CE, cholesteryl ester; PL, phospholipids.