A comparative structural analysis of the surface properties of asco-laccases
Fig 5
Phenolic substrate binding pockets in asco-type laccases.
A) Superposition of MtL (blue) and TaL (3PPS, green) with the MaL:2,6-DMP complex (3FU7, grey). MtL residues lining the pocket are labelled. Glu235 and His508 that comprise the polar recognition motif are highlighted with red and blue bars, respectively. B) Superposition of BaL (3SQR, yellow) and AnL (5LM8, orange). The polar recognition motif and the extra polar residue, Glu497 (BaL numbering), are indicated. C)—G) Surface representations illustrating the topology of the T1-pockets and mapping of polar residues. Color-coding as in A)—B). H) Structural superposition shown as Cα-traces illustrating variations in loops around the T1-pocket. The ligand 2,6-DMP (black spheres) from 3FU7 indicates the location of the T1-pocket.