Influence of bradykinin B2 receptor and dopamine D2 receptor on the oxidative stress, inflammatory response, and apoptotic process in human endothelial cells
Fig 7
Relative caspase 3/7 activity in endothelial cells after treatment with B2R and D2R agonists.
Cells (1.0 × 104) without (A) preincubation or (B) preincubated with 10 ng/ml TNF-α for 6 hours were treated with 100 nM BK, 100 nM SUM, or both agonists simultaneously for 24 hours. Enzyme activity was analyzed with a chemiluminescent assay. The figures present the mean values ± SD of the percentage change in caspase 3/7 activity compared with the untreated cells (with an assumed reference control value of 100%; dashed line). The results were obtained from at least three experiments performed in triplicate. &P < 0.001 versus untreated cells, *P < 0.001 versus BK-treated cells, #P < 0.001 versus SUM-treated cells.