Influence of bradykinin B2 receptor and dopamine D2 receptor on the oxidative stress, inflammatory response, and apoptotic process in human endothelial cells
Fig 6
Effect of B2R and D2R agonists on the expression of pro- and anti-apoptotic proteins.
Cells (1.0 × 106) were treated with 100 nM BK, 100 nM SUM, or both agonists simultaneously for 6 or 24 hours. (A) The protein expression levels of Bcl-2, Bcl-xL, and Bax was analyzed by western blotting. The (B) Bcl-2/Bax and (C) Bcl-xL/Bax ratios were calculated by densitometric analysis and the values were normalized to the β-actin expression level. The figures represent the mean values ± SD from two experiments, compared with the value obtained for the untreated cells, assumed to be 1 (dashed line). &P < 0.001 versus untreated cells, * P < 0.001 versus BK-treated cells, #P < 0.005 versus SUM-treated cells.