Influence of bradykinin B2 receptor and dopamine D2 receptor on the oxidative stress, inflammatory response, and apoptotic process in human endothelial cells
Fig 2
ROS production by HUV-EC-Cs treated with B2R and D2R agonists.
DCFH-DA-loaded endothelial cells (1.0 × 105) were stimulated with 100 nM BK, 100 nM SUM, or both agonists simultaneously. Some samples were additionally preincubated with 10 μM HOE 140 or 10 μM eticlopride for 30 minutes or 1 μM apocynin for 1 hour prior to agonist stimulation. The bars represent the mean percentage of the changes in ROS production relative to the untreated cells (with an assumed reference control value of 100%; dashed line). The more significant differences were indicated inside figure. *P < 0.001 versus BK-treated cells, #P < 0.001 versus SUM-treated cells.