The BH3 only Bcl-2 family member BNIP3 regulates cellular proliferation
Fig 3
Loss of BNIP3 increased proliferation in cultured primary astrocytes.
(A) Equivalent quantities of wild-type (WT) and BNIP3 knockout (KO) astrocytes isolated from neonatal brains of mice were seeded in 6-well plates, and plates were analyzed using the Cytation V instrument. The cell count shown is an average of three independent wells. (B) Equivalent numbers of wild type (WT) and BNIP3 knockout (KO) astrocytes were plated in triplicate in 96-well plates and allowed to replicate for 1 to 6 days. Relative cell density was measured by quantifying the amount of MTT reagent metabolized to purple formazan in each well by colorimetric assay. The error bars show standard error of the mean. Student’s t-test was used to calculate statistical significance. (ns: not significant, *: p<0.05, **: p<0.01).