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Resveratrol enhances the clearance of mitochondrial damage by vitrification and improves the development of vitrified-warmed bovine embryos

Fig 5

Effect of resveratrol on the ROS content in blastocysts and blastomeres.

Vitrified-warmed embryos were cultured with either vehicle (control) or resveratrol (0.5μM), and the blastocysts were stained with CellROX. A) ROS content per blastocyst, B) ROS content per blastomere, C) Representative images of CellROX-stained blastocysts. The average fluorescence intensity of vitrified-warmed embryos cultured with vehicle (control) was defined as 1.0. Bars with different letters represent difference between treatments (P < 0.05). Data are presented as mean ± SEM.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0204571.g005