Conformational plasticity of the intrinsically disordered protein ASR1 modulates its function as a drought stress-responsive gene
Fig 2
A) CD spectrum changes for ASR1 (2 μM) after the addition of increasing amounts of ZnCl2; inset: Mean Residual Molar Ellipticity at 222 nm and % of calculated α-helix as a function of Zn2+ concentration. The solid line corresponds to the fit of 1:1 binding model (Eq 5). B) Elution profile of ASR1 (63 μg) by SEC using an analytical Superdex 75 column: without Zn2+ (full line) and with 10 μM Zn2+ (dash line); inset: Molar weight determination by SLS for the protein without Zn2+ addition. C) DLS measurements of 40 μM ASR1 with 0, 5 and 10 μM Zn2+. Frequency histogram of hydrodynamic radius (logarithmic scale) is plotted with the corresponding standard deviation.