Dynamic and tissue-specific proteolytic processing of chemerin in obese mice
Fig 6
Chemerin levels in adipose tissue extracts of mice fed a low-fat or high-fat diet.
Total chemerin in brown fat extracts (A) and epididymal fat extracts (B), mchem162K, mchem155F and mchem154A in epididymal fat extracts (C, D, and E) of 12 weeks old mice fed with either a LF (designated with a blue circle) or HF (designated with a red square) diet, and 26 weeks old mice fed with either a LF (designated with a blue triangle) or HF (designated with a red triangle) diet were determined by ELISA. Horizontal lines show the mean ± SEM. (n = 10–14). There was no mchem157R and mchem156S detected in epididymal fat extracts. (F) chemerin forms in epididymal fat extracts presented as a percentage of the sum of the five specific ELISAs. Relationship between epididymal fat extracts total chemerin and weight (G), body fat % (H), or plasma total chemerin (I), brown fat extracts total chemerin and weight (J), body fat % (K), or plasma total chemerin (L) (n = 47), multigroup comparisons were by ANOVA followed by Kruskal-Wallis analysis. **: p<0.01; *** p<0.001 ****: p<0.0001. The Pearson correlation coefficient was calculated with a two-tailed p value.