Dynamic and tissue-specific proteolytic processing of chemerin in obese mice
Fig 5
Chemerin levels in plasma of mice fed a low-fat or high-fat diet.
Total chemerin (A), mchem162K (B), mchem157R (C), mchem156S (D), mchem155F (E), and mchem154A (F) levels in plasma of 12 weeks old mice fed with either a low-fat (LF) (designated with a blue circle) or high-fat (HF) (designated with a red square) diet, and 26 weeks old mice fed with either a LF (designated with a blue triangle) or HF (designated with a red triangle) diet were determined using total chemerin ELISA and specific ELISAs. Horizontal lines show the mean ± SEM. (n = 10–14). (G) chemerin forms in plasma presented as a percentage of the sum of the five specific ELISAs (mchem162K + mchem157R + mchem156S + mchem155F + mchem154A). Comparisons were by ANOVA followed by post-hoc Kruskal-Wallis analysis with two-tailed p value. *: p<0.05; **: p<0.01; ****: p<0.0001. Relationship between mouse plasma total chemerin and weight (H), plasma total chemerin and body fat % (I), plasma mchem162K and weight (J), and plasma mchem162K and body fat % (K) (n = 47). The Pearson correlation coefficient was calculated with a two-tailed p value.