Dynamic and tissue-specific proteolytic processing of chemerin in obese mice
Fig 3
Characterization of specific antibodies against chemerin forms.
(A) purified antibodies specific for different chemerin forms were characterized by western blot analysis of mchem161T (lane 1), mchem157R (lane 2), mchem156S (lane 3), mchem155F (lane 4), and mchem154A (lane 5) with anti-mchem162K, anti-mchem157R, anti-mchem156S, anti-mchem156F, and anti-mchem154A. Molecular mass markers are shown on the left of the panels. (B) mchem161T (blue), mchem157R (red), mchem156S (green), mchem155F (purple) and mchem154A (brown) were detected with specific ELISAs using anti-mchem162K, anti-mchem157R, anti-mchem156S, anti-mchem155F, and anti-mchem154A respectively. Bottom right panel displays the results of mchem161T (blue), mchem157R (red), mchem156S (green), mchem155F (purple) mchem154A (brown) and R&D standard (black) in the R&D total mouse chemerin ELISA.