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Nanoplasmonic pillars engineered for single exosome detection

Fig 3

Spectral LSPR data versus LSPRi.

(A) LSPR spectra from a 400 nanopillar array taken before (black) and after (red) introducing the exosomes (105 exosomes/ml). The spectrometer’s spatial resolution and integration time were 200 μm2 and 5 sec, respectively (B) CMOS Image of the same array allowing for single nanopillar resolution, 0.5 μm2 resolution, and a faster integration time of 1.3 sec. Grid overlay denotes region of interest for individual nanopillar intensity measurements. In LSPRi the nanopillars brighten in response to analyte as a result of the shifting spectra. Scale bar = 1 um.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0202773.g003