Nanoplasmonic pillars engineered for single exosome detection
Fig 2
MCF7 exosome characterization.
(A) Western blot analysis of exosomes (i) MCF7 exosome protein profile (ii) Colorimetric image (CA) lane displays standard weight markers and chemiluminescent image (CL) lane shows CD63 detection near 55 kD. (iii) Colorimetric image (CA) lane displays standard weight markers and chemiluminescent image (CL) lane shows CD9 detection near 28 kD. (B) Nanosight analysis of MCF7 exosomes showing the size distribution. (C) AFM image of a single MCF7 exosomes in 0.1 M PBS on freshly cleaved mica. (D) AFM image of larger populations of MCF7 exosomes. (E) Representative cross-sections of several exosomes taken from AFM scans.