Nanoplasmonic pillars engineered for single exosome detection
Fig 1
(A) 25.4 mm diameter LSPRi sensor chip. (B) LSPRi image of a 20 × 20 array, with a pitch size of 600 nm scale bar: 1 μm. (C) LSPRi image of sixteen arrays in the FOV taken using 100X / 1.4 NA objective, each consisting of 400 plasmonic nanopillars in a 20 × 20 square lattice and 500 nm pitch, scale bar: 10 μm. (D) False colored SEM image of a 10 × 10 nanopillar array, scale bar: 1 μm. (E) High-magnification false colored SEM image showing detailed view of individual nanopillars, scale bar: 200 nm. (F) Diagram illustrating size matching of individual nanopillars diameter (d = 90 nm) to that of exosomes (~50 nm < d < 200 nm), allowing digitized exosome detection while also elevating the sensor to minimize background contributions from the substrate.