Intrinsic proteotoxic stress levels vary and act as a predictive marker for sensitivity of cancer cells to Hsp90 inhibition
Fig 5
Correlation of HSF1 basal activity with sensitivity characterised by Hsp70/CHIP ratio to Hsp90 inhibitors.
(A-C) HSF1 activity expressed as the ratio between the HSF1 regulated co-chaperone Hsp70 and the HSF1 independent co-chaperone CHIP correlated with IC20 (nM) of the indicated Hsp90 inhibitors. (A) NVP-AUY922 (r = -0.711; p = 0.0171), (B) BIIB021 (r = -0.660; p = 0.0265) and (C) AT13387 (r = -0.591; p = 0.0435). (D) mRNA copy number of analysed genes per reaction. qPCR measurements were performed from 3 biological replicates.