Intrinsic proteotoxic stress levels vary and act as a predictive marker for sensitivity of cancer cells to Hsp90 inhibition
Fig 4
Correlation of HSF1 basal activity characterised by HOP/CHIP ratio with sensitivity to Hsp90 inhibitors.
(A-C) HSF1 activity expressed as the ratio between the HSF1 regulated co-chaperone HOP and the HSF1 independent co-chaperone CHIP correlated with IC20 (nM) of the indicated Hsp90 inhibitors. (A) NVP-AUY922 (r = -0.707; p = 0.0178), (B) BIIB021 (r = -0.602; p = 0.0402) and (C) AT13387 (r = -0.530; p = 0.0637). (D) mRNA copy number of analysed genes per reaction. qPCR measurements were performed from 3 biological replicates.