Intrinsic proteotoxic stress levels vary and act as a predictive marker for sensitivity of cancer cells to Hsp90 inhibition
Fig 2
Analysis of HSF1 in response to NVP-AUY922.
(A) Western blot analysis of phosphorylated HSF1 (Ser326) and total HSF1 in breast cancer cell lines. A representative blot is shown of 3 biological replicates. (B) Graph shows increase in ratio of pSer326HSF1 to total HSF1 after 4 h treatment with 200 nM NVP-AUY922 correlated to IC20 (nM) values of NVP-AUY922 treated breast cancer cell lines (r = 0.718; p = 0.0161).