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Manchette-acrosome disorders and testicular efficiency decline observed in hypercholesterolemic rabbits are recovered with olive oil enriched diet

Fig 14

Sperm elongation complex model.

The nucleus is represented in red, the acrosome in light blue, the acrosomal granule in blue, the marginal ring with a blue line, the perinuclear ring with a dark green line, the microtubules of manchette with light green lines. The space between acrosome and nucleus corresponds to acroplaxome, and the space between marginal and perinuclear ring to circumferential groove. (A) HCARDA spermatid. The lipid droplet could disturb the acrosomal granule position and modify the elongation axis. The acrosome is lax, the acroplaxome and the marginal ring are disrupted. The whorls in the circumferential groove may affect marginal and perinuclear ring interaction. The manchette seems to pull asymmetrically. (B) ½ HCARDA + ½ OO spermatid. The cytoplasm is free of whorls and lipid droplets. The acrosome granule is positioned equidistantly from both edges of the acrosome. The interaction between marginal and perinuclear rings appears to be recovered. Finally, the acrosome is well positioned on the nucleus and symmetrically pulled by the manchette. This elongation complex is similar to normal.

Fig 14

doi: https://doi.org/10.1371/journal.pone.0202748.g014