Manchette-acrosome disorders and testicular efficiency decline observed in hypercholesterolemic rabbits are recovered with olive oil enriched diet
Fig 13
Detection of apoptosis by TUNEL assay in isolated spermatogenic cells.
(A) Left column (a, d and g: green signal) corresponds to TUNEL-positive spermatogenic cells, middle column (b, e and h: red signal) to nucleus detection by propidium iodide, and right column (c, f and i) to merge. TUNEL-positive cells are surrounded by a white circle. Magnification: 400X. (B) Mean ± SD of TUNEL-positive cells (%) were plotted for NCR = black bar, HCARDA = white bar, and ½ HCARDA + ½ OO = grey bar. n = 200 cells per experimental condition. Asterisk = p< 0.05.