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Manchette-acrosome disorders and testicular efficiency decline observed in hypercholesterolemic rabbits are recovered with olive oil enriched diet

Fig 9

Ultra-structure of round spermatids.

NCR (left column, A and D): Acrosome (Ac), acrosome granule (AG) located symmetrically from both acrosomal borders, perinuclear ring (arrow) and microtubules (m). HCARDA (middle column, B and E): No detection of acrosomal symmetry, perinuclear or manchette arriving to the ring. Moreover, lax and asymmetric acrosome (B), and several vesicles and whorl (E) could be observed under HCARDA condition. ½ HCARDA + ½ OO (right column, C and F): recovered acrosome ultra-structure and symmetry without vesicles or whorls. Magnification: 10.000X (A, B and C, scale bar in A = 2 μm) and 40.000X (D, E and F, scale bar in D = 0.5 μm).

Fig 9

doi: https://doi.org/10.1371/journal.pone.0202748.g009