Zebrafish snai2 mutants fail to phenocopy morphant phenotypes
Fig 2
Predicted genomic and protein effects of snai2 morpholinos.
Location of the splice-blocking and translation-blocking morpholinos are indicated on a schematic of the snai2 gene (A). The green arrow indicates the transcription start site, and the red line the endogenous stop codon. Predicted effects on the amino acid sequence (B) and protein structure (C) are shown for wild-type and SB morphant fish. 121 bp of exon 2 are lost due to the aberrant splicing event, leading to a truncation of the protein within the zinc-finger domain. Italicism of the amino acid sequence and the grey region of the structure indicate a region of missense amino acids prior to the early stop codon. E: exon; SB: splice-block; MO: morpholino.