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High-throughput characterisation of bull semen motility using differential dynamic microscopy
Fig 7
A comparison of tracking and DDM methods for a sample maintained at 37°C for 15 min and 50 min after thawing.
(a,b) swimming speed distributions. Histogram of VAP (black) and VSL (grey) were calculated from 10 movies (10×, 100 fps, 500 p, 0.5 s) with ≈ 50 swimming tracks per movie. A lower magnification movie (2×, 300 fps, 500 p, 10 s) was recorded immediately afterwards and analysed with DDM to give 
(red vertical bar) and σ from which P(v) was reconstructed (red dashed line). Histograms are normalised such that the peak is at 1. (c,d) Head oscillation amplitude A0 and frequency f0 measured with DDM and tracking for consecutive movies of the same sample. Note that the quoted error for tracking is the standard deviation for measurements from 50 tracks, while that for DDM is a standard deviation of the mean for averaging over the values measured over a range of q values.
doi: https://doi.org/10.1371/journal.pone.0202720.g007