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High-throughput characterisation of bull semen motility using differential dynamic microscopy

Fig 6

Fitted parameters over the range q = 0-0.4 μm⁻¹.

(a) Mean and (b) width σ of the fitted Schultz swimming speed distribution, (c) proportion of motile cells α, (d) head amplitude A₀, (e) head frequency f₀ and (f) diffusion coefficient of the non-motile spermatozoa D_nm. (○) Fresh ejaculate diluted to ∼ 20 × 10⁶ cells/ml and recorded using (2.5×, 100 fps, 480 p, 40 s). These parameters correspond to the data in Fig 4. (□) thawed semen undiluted (∼ 80 × 10⁶ cells/ml) and recorded using (2×, 300 fps, 300 p, 40 s). (△) thawed semen diluted 4× to ∼ 20 × 10⁶ cells/ml recorded using (2×, 1000 fps, 300 p, 8 s).

doi: https://doi.org/10.1371/journal.pone.0202720.g006