The single mitochondrion of the kinetoplastid parasite Crithidia fasciculata is a dynamic network
Fig 6
Mitochondrial division occurs just prior to cytokinesis in C. fasciculata.
A) Time-lapse of mitochondrial/cell division in a swimming nectomonad immobilized in low melting point agarose. Image is a max projection of a Z-stack generated on a spinning disk confocal microscope and subjected to deconvolution. Times are given in mm:ss and numbers indicate the sequence of frames. The anterior of the cell is on the left side of each image. Complete mitochondrial division seems to occur between frames 3 and 4. A fenestrated sheet appears in the lower cell in frames 5 and 6 (arrowheads). Not all frames are shown (see S5 Movie). Scale bar is 2 μm. B) Mitochondrial and cell division of adherent haptomonads. Arrowheads indicate a dividing cell. Times are given in mm:ss and numbers indicate the sequence of frames. Not all frames are shown (see S6 Movie). Scale bar is 10 μm. C) Time lapse of a dividing haptomonad. The presumed position of the kinetoplast (identified by the small loop of mitochondrial matrix surrounding it, is indicated by white arrows. Numbers indicate the sequence of frames. Not all frames are shown (see S8 Movie). kDNA division occurs in frame 6–7. Scale bar is 2 μm.