The single mitochondrion of the kinetoplastid parasite Crithidia fasciculata is a dynamic network
Fig 5
Adherent C. fasciculata haptomonads also show rapid mitochondrial remodeling.
A) Max projections of a typical rosette of C. fasciculata imaged using a laser scanning confocal microscope. Mitochondria in live cells were visualized using mitoGFP. Mitochondrial remodeling reactions are indicated by arrows. Times are given as mm:ss. White numbers indicate the sequence of frames. Note that not every frame is shown (see S3 Movie). Scale bar is 10 μm. B) Time course of mitochondrial remodeling in a single adherent cell. Each frame is a max projection that has been color-coded by depth. White numbers indicate the sequence of frames. Note that not every frame is shown (see S4 Movie). Bracket indicates the formation of a net-like structure that then is rapidly remodeled to tubules during the course of our observation. Scale bar is 5 μm.