The single mitochondrion of the kinetoplastid parasite Crithidia fasciculata is a dynamic network
Fig 4
Mitochondrial dynamics in C. fasciculata.
A) Frames from live-cell imaging of two cells by spinning-disk confocal microscopy. Cells are expressing mitoGFP. Mitochondrial remodeling reactions are indicated by arrows. Times are given as h:min:s. White numbers indicate frame sequence. Not all frames in the time-course are shown (see S1 and S2 Movies) Scale bar is 5 μm. B) Quantitation of mitochondrial remodeling events in spinning disk confocal experiments. Z-stacks of 17 cells were generated every 2 min for an average of 49 min. The resulting movies were then analyzed for different types of mitochondrial remodeling events. The frequency of each event per minute was calculated then multiplied by 30 to give number of events/30 min time period. The bar labeled total represents the sum of all the different types of events shown by the other bars. C) Live-cell imaging of mitochondrial remodeling with a laser-scanning confocal microscope and deconvolution. White numbers indicate different frames. Boxes indicate areas in which remodeling occurs between frames. These areas are shown enlarged (with corresponding numbers) in the right panels. Scale bars are 2 μm.