The single mitochondrion of the kinetoplastid parasite Crithidia fasciculata is a dynamic network
Fig 1
mitoGFP is a mitochondrial marker in C. fasciculata.
A) Mitochondrial staining by the potential-dependent dye MitoTracker Red (MitoTracker, top panel) and by mitochondrial-targeted GFP (mitoGFP, lower panel). mitoGFP expression is driven by an ectopic plasmid causing heterogeneous fluorescence among cells. Scale bar is 10 μm. B) Higher magnification images of two representative cells show colocalization between MitoTracker and mitoGFP signals. The phase contrast image shows the cell boundaries. Nuclear DNA (arrowhead) and mitochondrial DNA (kDNA, arrow) are stained with DAPI. In merged images MitoTracker is shown in magenta and mitoGFP in green. Scale bar is 5 μm.