Expression and contribution to virulence of each polysaccharide capsule of Bacillus cereus strain G9241
Fig 6
The HA capsule was extracted from the cell surface with chloroform from 2 ml G9241, ΔwchA, Δwzg, ΔhasACB, ΔwchAΔhasACB, and pBCXO1-/pBC210- cells at OD600 0.34–0.36. The quantity of HA capsule in the extract was determined by Stains-all assay and comparison against a standard curve of purified HA. Three strains (ΔhasACB, ΔwchAΔhasACB and pBCXO1-/pBC210-) had no detectable HA by this assay. Only ΔhasACB values are shown on the graph. The bars indicate the mean of four experiments and the error bars the standard deviation. * Significantly different (P < 0.0003) from G9241 and Δwzg as determined by one-way ANOVA with Tukey’s multiple comparisons test.