Expression and contribution to virulence of each polysaccharide capsule of Bacillus cereus strain G9241
Fig 4
Promoter activity for wchJ, wzg, and wzd.
Fragments proximal to the start codon for wchJ (160, 349, and 607 bp), wzg (871 bp), and wzd (40, 310, and 502 bp) were cloned before the promoterless lacZ in pHT304-18Z in strain ΔwchAΔhasACB. The cells were grown under capsule-inducing conditions for 4 h, and the β-galactosidase activity was measured. The bars indicate the mean of four experiments and the error bars the standard deviation. *Significantly different from empty vector control as determined by one-way ANOVA with Dunnett’s multiple comparisons test (P < 0.0001).