Curcumin mediates polyamine metabolism and sensitizes gastrointestinal cancer cells to antitumor polyamine-targeted therapies
Fig 6
AGS (A) or HCT116 (B) cells were treated with curcumin for 48 hours in the presence or absence of spermidine or spermine and the bovine serum amine oxidase inhibitor aminoguanidine. MTS assays revealed that exogenous polyamines failed to protect cells from the growth inhibitory effects of curcumin. Data points indicate the means of at least 2 individual experiments, each measured in triplicate, with error bars representing SEM. (C) HPLC analysis of cotreated AGS cells confirmed uptake of spermine and spermidine into the cells, indicating that curcumin did not interfere with polyamine transport. Columns depict the means; error bars indicate SEM; n = 2, each measured in duplicate.