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Curcumin mediates polyamine metabolism and sensitizes gastrointestinal cancer cells to antitumor polyamine-targeted therapies

Fig 3

Curcumin induces spermine catabolism through spermine oxidation in AGS cells.

AGS cells were treated for 48 hours and analyzed for SMOX (A) and SSAT (D) mRNA expression via qRT-PCR; (B-C) SMOX and SSAT protein levels by Western blot; and (E) SSAT enzyme activity. Graphs depict the mean values of at least 2 individual experiments, each measured in triplicate. Error bars = SEM; *p < 0.05. A representative western blot is presented in (C).

Fig 3

doi: https://doi.org/10.1371/journal.pone.0202677.g003